Co-adaptor driven assembly of a CUL3 E3 ligase complex
David Akopian, Colleen A. McGourty, Michael Rapé
Abstract
as a model, we found that its co-adaptor PEF1-ALG2 initiates CRL3 formation by releasing KLHL12 from an assembly inhibitor at the endoplasmic reticulum, before co-adaptor monoubiquitylation stabilizes the enzyme for substrate modification. As the co-adaptor also helps recruit substrates, its role in CRL assembly couples target recognition to ubiquitylation. We propose that regulators dedicated to specific CRLs, such as assembly inhibitors or co-adaptors, cooperate with target-agnostic adaptor exchange mechanisms to establish E3 ligase complexes that control metazoan development.
Topics & Concepts
Signal transducing adaptor proteinUbiquitin ligaseCullinBiologyCell biologyUbiquitinDNA ligaseEndoplasmic reticulumUbiquitin-Protein LigasesSignal transductionBiochemistryEnzymeGeneUbiquitin and proteasome pathwaysAutophagy in Disease and TherapyGenetics and Neurodevelopmental Disorders