Extraction, structural characterization, and the potential against H1N1 influenza virus of the polysaccharide from Polygonatum sibiricum
Haoran Wu, Liang Li
Abstract
Polysaccharides are the key functional components in Polygonatum sibiricum . The aims of this investigation were to improve the extraction process, prepare the purified polysaccharide, explore its structural characteristics, and assess its potential against the H1N1 influenza virus. The optimal extraction conditions were obtained as follows: an extraction temperature of 86 °C, an extraction duration of 61 min, and a liquid-solid ratio of 25 mL/g. A pure polysaccharide, designated PSF, was extracted from P. sibiricum using water extraction, followed by precipitation with ethanol and chromatographic techniques. Its structural properties were examined and clarified by methylation assay, GC-MS, HPAEC, FT-IR, and NMR methods. According to the acquired data, PSF was identified as a neofructan characterized by a backbone of →1)-β-D-Fru f- (2→ with an internal →6)-α-D-Glc p- (1→, having the branches of →6)-β-D-Fru f- (2→ and β-D-Fruf-(2→ residues. It could effectively alleviate the damage of lung in mice infected with H1N1virus, and the mechanisms were related to the modulation of lung inflammatory factor levels and the balance of CD4 + and CD8 + lymphocytes' proportion in the spleen, indicating its potential as a novel therapeutic agent for viral infections. The findings established a research basis for deep development and utilization of P. sibiricum and its polysaccahrides.