Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization
Kristýna Dufková, Matěj Bezdíček, Kristina Cuprova, Dagmar Pantůčková, Markéta Nykrýnová, Eva Brhelová, Iva Kocmanová, Silvie Hodová, Markéta Hanslianová, Tomáš Juren, Břetislav Lipový, Jiřı́ Mayer, Martina Lengerová
Abstract
Rapid and cost-effective molecular typing tools for Staphylococcus aureus epidemiological applications such as transmission tracking, source attribution and outbreak investigations are highly desirable. High-resolution melting based methods are effective alternative to those based on sequencing. Their good reproducibility and easy performance allow prospective typing of large set of isolates while reaching great discriminatory power. In this study, we established a new epidemiological approach to S. aureus typing. This scheme has the potential to greatly improve epidemiological investigations of S. aureus.