Immunomodulatory effects of black solo garlic (Allium sativum L.) on streptozotocin-induced diabetes in Wistar rats
Saryono Saryono, Saryono, Desiyani Nani, Atikah Proverawati, Sarmoko, Sarmoko
Abstract
Diabetes mellitus is a chronic disease that leads to different complications. Therefore, this study aims to investigate the immunomodulatory effects of the black solo garlic on streptozotocin (STZ)-induced diabetic rats. The Wistar rats were grouped into six groups of: normal control, negative control, treatment dose of 6.5 g/kg, 13.5 g/kg, and 26 g/kg body weight, and positive control glibenclamide. In addition to normal control, rats were induced with STZ on day 8-11. Also, steeping black solo garlic or glibenclamide was administered on the day 12-19. The experimental animals were sacrificed on day 20 and tumor necrosis factor (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β) and interferon gamma (IFN-γ) were measured using ELISA. The results showed that the administration of steeping black solo garlic significantly decreased levels of IL-1β, IL-6 and TNF-α as well as increased IFN-γ with the immunity of STZ-induced rats.