Characterization of FRI carbapenemase-producing <i>Enterobacter</i> spp. isolated from a hospital and the environment in Osaka, Japan
Fumie Adachi, Tsuyoshi Sekizuka, Masaya Yamato, Kyoko Fukuoka, Nobuyasu Yamaguchi, Makoto Kuroda, Ryuji Kawahara
Abstract
The spread of antimicrobial-resistant bacteria is a global public health problem. Carbapenem-resistant Enterobacterales are major healthcare-associated pathogens that often cause outbreaks in hospitals.1 Genes encoding major carbapenemases, including IMP, VIM, NDM, KPC and OXA-48, have been increasingly detected, not only in clinical settings but also in the environment.2 French imipenemases (FRIs) are a recently discovered type of carbapenemase. FRI-1–3 were reported in Europe between 2015 and 2017.3–5 FRI-4–7 were reported in Japan and Canada between 2017 and 2019.6,7 The increasing number of reports about FRI variants suggest that FRI carbapenemases may be spreading across the world. Enterobacter asburiae 17Nkhm-UP2 and Enterobacter sp. 18A13 were isolated from a river into which effluent is discharged from a sewage treatment plant and E. asburiae 1808-013 was isolated from the urine of a patient. Drug susceptibility testing was performed using a broth microdilution method and MICs were interpreted...