Synergistic Effect of Antimicrobial Peptide LL-37 and Colistin Combination Against Multidrug-Resistant <i>Escherichia Coli</i> Isolates
Gianluca Morroni, Laura Di Sante, Oriana Simonetti, Lucia Brescini, Wojciech Kamysz, Elżbieta Kamysz, Marina Mingoia, Andrea Brenciani, Eleonora Giovanetti, Patrizia Bagnarelli, Andrea Giacometti, Oscar Cirioni
Abstract
Overview: The global spread of antibiotic resistance represents a serious threat for public health. Aim: We evaluated the efficacy of the antimicrobial peptide LL-37 as antimicrobial agent against multidrug-resistant Escherichia coli. Results: LL-37 showed good activity against mcr-1 carrying, extended spectrum β-lactamase- and carbapenemase-producing E. coli (minimum inhibitory concentration, MIC, from 16 to 64 mg/l). Checkerboard assays demonstrated synergistic effect of LL-37/colistin combination against all tested strains, further confirmed by time–kill and post antibiotic effect assays. MIC and sub-MIC concentrations of LL-37 were able to reduce biofilm formation. Conclusion: Our preliminary data indicated that LL-37/colistin combination was effective against multidrug-resistant E. coli strains and suggested a new possible clinical application.