Effects of polysaccharide-based silver and selenium nanoparticles on growth performance, biochemical parameters, and immune response of Cyprinus carpio
Natwar Jha, A.R. Annamalai, Palanichamy Essakiraj, Ramachandran Balamurugan, Avinash Kant Lakra, Younus Mohd Tilwani, Venkatesan Arul
Abstract
Avicennia marina mangrove leaves polysaccharide (AMLP) was used for the synthesis of polysaccharide-based selenium (AMLP-SeNPs) and silver nanoparticles (AMLP-AgNPs). The synthesized nanoparticles were further characterized by UV-Vis, DLS, FT-IR, X-ray diffraction, and HR-TEM analysis. A 60-day (8 weeks) feeding trial experiment was conducted to investigate the effects of AMLP, AMLP-SeNPs, and AMLP-AgNPs dietary supplementation on growth performance parameters, blood parameters, immunological and enzymatic profiles in Cyprinus carpio. The characterization results of AMLP-SeNPs and AMLP-AgNPs confirmed the formation of well-stabilized spherical nanoparticles with a mean particle size of 37.25 and 72.40 nm, respectively having a crystalline structure. The feeding experiment results demonstrated that 2 mg/kg of AMLP-SeNPs followed by 0.2 mg/kg of AMLP-AgNPs showed significantly (p ˂ 0.05) higher final weight, weight gain (WG), specific growth rate (SGR%), protein and lipid efficiency, and lower food conversion ratio as compared to other groups. The catalase, superoxidase dismutase, and glutathione peroxidase activity were significantly (p ˂ 0.05) higher in the group fed 2 mg/kg supplemented AMLP-SeNPs. Total protein and globulin contents were significantly (p ˂ 0.05) higher and albumin concentration was significantly lower in fish that received 2 mg/kg of AMLP- SeNPs as compared to control. A significant increase in serum HDL and decrease in LDL and MDA concentrations were observed in the group supplemented with 2 mg/kg of nano selenium. The body's crude lipid, protein, moisture, and ash were not significantly different from the control. The AMLP-SeNPs showed significantly (p ˂ 0.05) lower aspartate aminotransferase (AST), alanine aminotransferase (ALT), and higher alkaline phosphatase (ALP) activities compared to other test groups. The relative percentage survivability (RPS%) was higher in AMLP-SeNPs (84.6%) followed by AMLP-AgNPs (76.7%) after 8th weeks of supplementary diets as compared to control groups. Overall, the finding of these studies revealed that the inclusion of AMLP-SeNPs improved the growth performance and antioxidant defense system, enhance immune response, and provide resistance against Aeromonas hydrophila in Common carp.