Rational Construction of a Two-Photon NIR Ratiometric Fluorescent Probe for the Detection of Bisulfite in Live Cells, Tissues, and Foods
Jingcan Qin, Fei Kong, Yuanyuan Guo, Dongya Wang, Chuan Zhang, Yuehua Li
Abstract
In this study, we report a novel ratiometric fluorescent probe with a blue shift of 180 nm based on a D-π-A-A structure. The probe composed of a hydroxyl moiety as a donor, a naphthyl ring as a π bridge, and benzothiazole/hemicyanine as an acceptor has good selectivity and high sensitivity to bisulfite (HSO3–) in aqueous solution. Besides one-photon fluorescence properties, the probe possesses excellent two-photon fluorescence properties and is successfully utilized for fluorescence imaging of HSO3– in MCF-7 cells and rat liver tissues. More importantly, the probe also has practical application potential for measuring the HSO3– content of real food samples.
Topics & Concepts
FluorescenceBisulfiteBenzothiazoleMoietyChemistryPhotochemistryTwo-photon excitation microscopyAbsorption (acoustics)SelectivityStereochemistryMaterials scienceOrganic chemistryBiochemistryCatalysisOpticsComposite materialGenePhysicsGene expressionDNA methylationSulfur Compounds in BiologyObstructive Sleep Apnea ResearchAdvanced Glycation End Products research