Development of Benzimidazole‐Substituted Spirocyclopropyl Oxindole Derivatives as Cytotoxic Agents: Tubulin Polymerization Inhibition and Apoptosis Inducing Studies
Akash P. Sakla, Mohd Rabi Bazaz, Ashutosh Mahale, Pravesh Sharma, Durgesh Gurukkala Valapil, Onkar Prakash Kulkarni, Manoj P. Dandekar, Manoj P. Dandekar, Nagula Shankaraiah
Abstract
Abstract A series of spirocyclopropyl oxindoles with benzimidazole substitutions was synthesized and tested for their cytotoxicity against selected human cancer cells. Most of the molecules exhibited significant antiproliferative activity with compound 12 p being the most potent. It exhibited significant cytotoxicity against MCF‐7 breast cancer cells (IC 50 value 3.14±0.50 μM), evidenced by the decrease in viable cells and increased apoptotic features during phase contrast microscopy, such as AO/EB, DAPI and DCFDA staining studies. Compound 12 p also inhibited cell migration in wound healing assay. Anticancer potential of 12 p was proved by the inhibition of tubulin polymerization with IC 50 of 5.64±0.15 μM. These results imply the potential of benzimidazole substituted spirocyclopropyl oxindoles, notably 12 p , as cytotoxic agent for the treatment of breast cancer.