Metformin inhibits gastric cancer cell proliferation by regulation of a novel Loc100506691-CHAC1 axis
Hui‐Hwa Tseng, You-Zuo Chen, Nan–Hua Chou, Yen-Chih Chen, Chao‐Chuan Wu, Lifeng Liu, Yi‐Fang Yang, Chung-Yu Yeh, Mei‐Lang Kung, Yating Tu, Kuo‐Wang Tsai
Abstract
mouse model. Analysis of the cell cycle revealed that Loc100506691 knockdown induced cell cycle arrest at the G2/M phase by impairing cell entry from the G2/M to G1 phase. Loc100506691 negatively regulated CHAC1 expression by modulating miR-26a-5p/miR-330-5p expression, and CHAC1 knockdown markedly attenuated Loc100506691 knockdown-induced gastric cancer cell growth and motility suppression. We concluded that anti-proliferative effects of metformin in gastric cancer may be partially caused by suppression of the Loc100506691-miR-26a-5p/miR-330-5p-CHAC1 axis.
Topics & Concepts
Gene knockdownCancerCell growthEctopic expressionCancer researchCell cycleMetforminCancer cellBiologyCellCell cycle checkpointInternal medicineEndocrinologyMedicineCell cultureInsulinBiochemistryGeneticsCancer-related molecular mechanisms researchRNA modifications and cancerMicroRNA in disease regulation