Comment on “Reintroduction of the archaic variant of <i>NOVA1</i> in cortical organoids alters neurodevelopment”
Tomislav Maričić, Nelly Helmbrecht, Stephan Riesenberg, Dominik Macak, Philipp Kanis, Martin Lackner, Agrafena Daria Pugach-Matveeva, Svante Pääbo
Abstract
. (Research Articles, 12 February 2021, eaax2537) conclude that the reintroduction of an ancestral amino acid substitution in the protein NOVA1 drastically alters the development of brain organoids. We show that cell lines used by the authors carry heterozygous deletions of the target DNA sequence, providing another plausible explanation for the effects observed.
Topics & Concepts
OrganoidBiologyEvolutionary biologyGeneticsComputational biologyRNA Research and SplicingCRISPR and Genetic EngineeringSingle-cell and spatial transcriptomics