Comparison of Five Commercial Molecular Assays for <i>Mycoplasma</i> Testing of Cellular Therapy Products
Arthur H. Totten, Anna Julia Adams, Hyunmi Halas, James E. T. Gebo, Amanda D. East, Anna F. Lau
Abstract
assay was most sensitive followed by the MycoSEQ and MycoTOOL which were comparable. We showed that not all assays were capable of meeting the ≤10 CFU/mL LOD to replace culture-based methods according to European and Japanese pharmacopeia standards. No assay interference was observed when testing in the presence of TILs.
Topics & Concepts
MycoplasmaRepeatabilityAgarSerial dilutionMicrobiologyMycoplasma genitaliumBiologyDetection limitVirologyChromatographyMedicineChemistryBacteriaPathologyChlamydia trachomatisGeneticsAlternative medicineMicrobial infections and disease researchBacteriophages and microbial interactionsHerpesvirus Infections and Treatments