Direct cell extraction of membrane proteins for structure–function analysis
Ieva Drulyte, Aspen Rene Gutgsell, Pilar Lloris-Garcerá, Michael A. Liss, Stefan Geschwindner, Mazdak Radjainia, Jens Frauenfeld, Robin Löving
Abstract
Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins directly from cells into lipid Salipro nanoparticles. Here, with the pannexin1 channel as a case study, we demonstrate the applicability of this method for structure-function analysis using SPR and cryo-EM.
Topics & Concepts
Membrane proteinMembraneCell membraneCellFunction (biology)Computational biologyChemistryCell biologyBiophysicsBiologyBiochemistryAdvanced Electron Microscopy Techniques and ApplicationsToxin Mechanisms and ImmunotoxinsLipid Membrane Structure and Behavior