An astrocyte cell line that differentially propagates murine prions
Waqas Tahir, Basant Abdulrahman, Dalia H. Abdelaziz, Simrika Thapa, Rupali Walia, Hermann Schätzl
Abstract
Interestingly, when we used prion conversion activity as a readout in real-time quaking-induced conversion assays with RML-infected cell lysates, we observed a strong signal over all six passages, comparable with that for 22L-infected cells. These data indicate that the C8D1A cell line is permissive to prion infection. Moreover, the propagated prions differed in conversion and proteinase K-resistance levels in these astrocytes. We propose that the C8D1A cell line could be used to decipher prion strain biology.
Topics & Concepts
Cell cultureBiologyAstrocyteGene isoformCell biologyIn vitroCellVirologyPrion proteinGenetically modified mouseMolecular biologyTransgeneGeneticsGeneDiseasePathologyNeuroscienceCentral nervous systemMedicinePrion Diseases and Protein MisfoldingNeurological diseases and metabolismTrace Elements in Health