Highly sensitive biosensor based on aptamer and hybridization chain reaction for detection of cadmium ions
Mingming Xu, Yu Peng, Hualin Yang, Yu Zhou
Abstract
Abstract In this work, a highly sensitive biosensor for detecting cadmium ions (Cd 2+ ) was developed based on a Cd 2+ ‐specific DNA aptamer and a hybridization chain reaction (HCR). The Cd 2+ aptamer (named S0) was used to recognize Cd 2+ and trigger the HCR. Without Cd 2+ , S0 initiated the HCR to form long nicked dsDNA structures to quench the fluorescence. Then, Cd 2+ could bind with S0 to block HCR to recover fluorescence. This biosensor had high sensitivity with a detection limit of 0.36 nM and a linear range from 0 to 10 nM. Moreover, it showed a satisfactory selectivity and recovery rates.
Topics & Concepts
AptamerBiosensorDetection limitFluorescenceChemistrySelectivityLinear rangeIonMetal ions in aqueous solutionChain reactionDNACadmiumHybridization probeCombinatorial chemistryAnalytical Chemistry (journal)PhotochemistryChromatographyMolecular biologyBiochemistryBiologyOrganic chemistryCatalysisPhysicsQuantum mechanicsAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionElectrochemical Analysis and Applications