Cautionary Notes on the Use of Arabinose- and Rhamnose-Inducible Expression Vectors in Pseudomonas aeruginosa
Emily A. Williams McMackin, Jodi M. Corley, Sardar Karash, Jeremiah N. Marden, Matthew C. Wolfgang, Timothy L. Yahr
Abstract
Heterologous gene expression and complementation constitute a valuable and widely utilized tool in bacterial genetics. The arabinose-inducible P araBAD (P BAD ) and rhamnose-inducible P rhaBAD (P Rha ) promoter systems are commonly used in P. aeruginosa genetics and prized for the tight control and dynamic expression ranges that can be achieved.
Topics & Concepts
BiologyRegulonPromoterrpoNTranscription factorHeterologousPseudomonas aeruginosaSigma factorEscherichia coliRegulation of gene expressionGeneticsGeneGene expressionBacteriaBacterial Genetics and BiotechnologyRNA and protein synthesis mechanismsChemical Synthesis and Analysis