Small-Molecule Activity-Based Probe for Monitoring Ubiquitin C-Terminal Hydrolase L1 (UCHL1) Activity in Live Cells and Zebrafish Embryos
Raymond Kooij, Sijia Liu, Ayşegül Sapmaz, Bo‐Tao Xin, George M. C. Janssen, Peter A. van Veelen, Huib Ovaa, Peter ten Dijke, Paul P. Geurink
Abstract
and in live cells. We furthermore show that this probe can selectively and spatiotemporally report UCHL1 activity during the development of zebrafish embryos. Our results indicate that our probe has potential applications as a diagnostic tool for diseases with perturbed UCHL1 activity.
Topics & Concepts
ChemistryZebrafishUbiquitinCysteineIn vitroCell biologyBiochemistryEnzymeDeubiquitinating enzymeSmall moleculeIn vivoBiologyGeneBiotechnologyUbiquitin and proteasome pathwaysProtein Degradation and InhibitorsHistone Deacetylase Inhibitors Research