Regulation of RB1CC1/FIP200 stability and autophagy function by CREBBP-mediated acetylation in an intrinsically disordered region
Fei Yi, Chunmiao Cai, Banzhan Ruan, Mingang Hao, Syn Kok Yeo, Michael Haas, Fuchun Yang, Xiaoting Zhang, Jun‐Lin Guan
Abstract
; CREBBP/CBP: CREB binding protein; CHX: cycloheximide; EP300/p300: E1A binding protein p300; FRAP: fluorescence recovery after photobleaching; HADCs: histone deacetylases; IDR: intrinsically disordered region; LLPS: liquid-liquid phase separation; KAT2A/GCN5: lysine acetyltransferase 2A; KAT2B/PCAF: lysine acetyltransferase 2B; KAT5/TIP60: lysine acetyltransferase 5; KAT8/MOF: lysine acetyltransferase 8; NAM: nicotinamide; PAS: phagophore assembly site; PEG-8000: polyethylene glycol 8000; RB1CC1/FIP200: RB1 inducible coiled-coil 1; TSA: trichostatin A.
Topics & Concepts
AcetylationCREB-binding proteinAutophagyAcetyltransferaseHistone acetyltransferasePCAFBiologyP300-CBP Transcription FactorsLysineCell biologyBromodomainUbiquitinATG12BiochemistryCREBHistone AcetyltransferasesAmino acidApoptosisTranscription factorGeneATG5Autophagy in Disease and TherapyRNA modifications and cancerUbiquitin and proteasome pathways