Development of a rapid pre-concentration protocol and a magnetic beads-based RNA extraction method for SARS-CoV-2 detection in raw municipal wastewater
Ana L. Parra Guardado, Crystal L. Sweeney, Emalie K. Hayes, Benjamin F. Trueman, Yannan Huang, Rob Jamieson, Jennie L. Rand, Graham A. Gagnon, Amina K. Stoddart
Abstract
We demonstrate the application of a rapid pre-concentration protocol and a magnetic bead-based RNA extraction method for the detection of SARS-CoV-2 RNA from raw wastewater without the need for extensive sample treatment.
Topics & Concepts
Extraction (chemistry)WastewaterMagnetic beadChromatographyRNA extractionRNARaw materialMagnetic nanoparticlesChemistryMaterials scienceWaste managementNanotechnologyNanoparticleBiochemistryEngineeringOrganic chemistryGeneSARS-CoV-2 detection and testingBiosensors and Analytical DetectionCOVID-19 diagnosis using AI