Mass Cytometry Phenotyping of Human Granulocytes Reveals Novel Basophil Functional Heterogeneity
Nora Vivanco Gonzalez, John Paul Oliveria, Dmitry Tebaykin, Geoffrey T. Ivison, Kaori Mukai, Mindy Tsai, Luciene Borges, Kari C. Nadeau, Stephen J. Galli, Albert G. Tsai, Sean C. Bendall
Abstract
(0.4 ± 0.1%). Prospective isolation confirmed basophilic-morphology of PBG I-III, but neutrophilic-morphology of PBG IV. Functional interrogation via IgE-crosslinking or IL-3 stimulation demonstrated that PBG I-II had significant increases in CD203c expression, whereas PBG III-IV remained unchanged compared with media-alone conditions. Thus, PBG III-IV could serve roles in non-IgE-mediated immunity. Our findings offer new perspectives in human basophil heterogeneity and the varying functional potential of these new subsets in health and disease.
Topics & Concepts
BasophilMass cytometryBasophilicImmunologyFlow cytometryBiologyCell sortingGranulocytePhenotypeMolecular biologyImmunophenotypingImmunoglobulin ECell biologyAntibodyPathologyMedicineBiochemistryGeneMast cells and histamineAsthma and respiratory diseasesAllergic Rhinitis and Sensitization