Quality-By-Design-Based Development of Ultradeformable Nanovesicular Transgelosome of Standardized <i>Piper Longum</i> Extract for Melanoma
Debadatta Mohapatra, Dulla Naveen Kumar, Singh Shreya, Dhananjay Panigrahi, Ashish Kumar Agrawal, Alakh N. Sahu
Abstract
Background: Melanoma is the most aggressive and deadly form of skin cancer. The stratum corneum of the skin is a major obstacle to dermal and transdermal drug delivery. Ultradeformable nanovesicular transferosome has the capacity for deeper skin penetration and its incorporation into hydrogel forms a transgelosome that has better skin permeability and patient compliance. Method: Here, the quality-by-design-based development and optimization of nanovesicular transgelosome of standardized Piper longum fruit ethanolic extract (PLFEE) for melanoma therapy are reported. Results: Compared with standardized PLFEE-loaded plain gel, the transgelosome displayed optimal pharmaceutical properties and improved ex vivo skin permeability and in vivo tumor regression in B16F10 melanoma-bearing C57BL/6 mice. Conclusion: The results reflect the potential of transgelosome for melanoma therapy.