Evaluating Whole Blood Clotting in vitro on Biomaterial Surfaces
Roberta M. Sabino, Ketul C. Popat
Abstract
on 2D biomaterials surfaces via a simple and fast hemolysis assay is presented. Whole human blood is placed onto the biomaterial surfaces and is allowed to clot for different time periods. After the specific time intervals, the surfaces are transferred into deionized (DI) water to release the free hemoglobin and the absorbance of this solution is measured. The absorbance value is proportional to the free hemoglobin concentration in the DI water due to lysis of red blood cells and gives an indirect correlation to the extent of blood clotting on the biomaterial surfaces. This protocol provides a fast, facile and effective method to measure the anti-thrombogenic properties of biomaterials.
Topics & Concepts
BiomaterialWhole bloodBiomedical engineeringThrombusHemolysisFibrinChemistryClotting timeCoagulationMaterials scienceSurgeryMedicineImmunologyInternal medicinePolymer Surface Interaction StudiesHemostasis and retained surgical itemsHemophilia Treatment and Research