A Leak-Free Inducible CRISPRi/a System for Gene Functional Studies in Plasmodium falciparum
Xiaoying Liang, Rachasak Boonhok, Faiza Amber Siddiqui, Bo Xiao, Xiaolian Li, Junling Qin, Hui Min, Lubin Jiang, Liwang Cui, Jun Miao
Abstract
. We evaluated the robustness and versatility of this system by activating or repressing eight selected genes and achieved up- and downregulation of the targeted genes located in both the euchromatin and heterochromatin regions. This system offers the malaria research community another tool for functional genetic studies.
Topics & Concepts
BiologyPlasmodium falciparumConditional gene knockoutMalariaGene knockdownComputational biologyGeneGene knockoutGeneticsImmunologyPhenotypeCRISPR and Genetic EngineeringMosquito-borne diseases and controlMalaria Research and Control