Leucine-enriched amino acids maintain peripheral mTOR-Rheb localization independent of myofibrillar protein synthesis and mTORC1 signaling postexercise
Sarkis J. Hannaian, Nathan Hodson, Sidney Abou Sawan, Michael Mazzulla, Hiroyuki Kato, Keiko Matsunaga, Marcus Waskiw‐Ford, Justin Duncan, Dinesh Kumbhare, Daniel R. Moore
Abstract
This is the first study to investigate whether postexercise leucine-enriched amino acid (LEAA) ingestion elevates mTORC1 translocation and protein-protein interactions in human skeletal muscle. Here, we observed that although LEAA ingestion did not further elevate postexercise MyoPS or mTORC1 signaling compared with placebo, mTORC1 peripheral location and interaction with Rheb were maintained. This may serve to "prime" mTORC1 for subsequent anabolic stimuli.
Topics & Concepts
mTORC1RHEBLeucineMyofibrilAmino acidPeripheralPI3K/AKT/mTOR pathwayEndocrinologyInternal medicineChemistryCell biologyProtein biosynthesisBiochemistryBiologySignal transductionMedicineMuscle metabolism and nutritionMuscle Physiology and DisordersGenetics and Physical Performance