Utility of Human<i>In Vitro</i>Data in Risk Assessments of Influenza A Virus Using the Ferret Model
Hannah M. Creager, Troy J. Kieran, Hui Zeng, Xiangjie Sun, Joanna A. Pulit-Penaloza, Katie E. Holmes, A. Johnson, Terrence M. Tumpey, Taronna R. Maines, Catherine A. A. Beauchemin, Jessica A. Belser
Abstract
Both in vitro and in vivo models are employed for assessing the pandemic potential of novel and emerging influenza A viruses in laboratory settings, but systematic examinations of how well viral titer measurements obtained in vitro align with results from in vivo experimentation are not frequently performed. We show that certain viral titer measurements following infection of a human bronchial epithelial cell line are positively correlated with viral titers in specimens collected from virus-inoculated ferrets and employ mathematical modeling to identify commonalities between viral infection progression between both models.