Proteomic analysis of microtubule inner proteins (MIPs) in Rib72 null <i>Tetrahymena</i> cells reveals functional MIPs
Amy S. Fabritius, Brian A. Bayless, Sam Li, Daniel Stoddard, Westley Heydeck, Christopher C. Ebmeier, Lauren Anderson, Tess Gunnels, Chidambaram Nachiappan, Justen B. Whittall, William M. Old, David A. Agard, Daniela Nicastro, Mark Winey
Abstract
axonemes. We identified a number of candidate MIPs and pursued one, Fap115, for functional characterization. We find that loss of Fap115 results in disrupted cell swimming and aberrant ciliary beating. Cryo-electron tomography reveals that Fap115 localizes to MIP6a in the A-tubule of the doublet microtubules. Overall, our results highlight the complex relationship between MIPs, ciliary structure, and ciliary function.
Topics & Concepts
TetrahymenaBiologyCiliumMicrotubuleAxonemeCell biologyFlagellumBasal bodyMotile ciliumCentrosomeCellBiochemistryGeneCell cycleMicrotubule and mitosis dynamicsProtist diversity and phylogenyGenetic and Kidney Cyst Diseases