An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells
Davi M. Lyra‐Leite, Hananeh Fonoudi, Mennat Gharib, Paul W. Burridge
Abstract
The protocol provided here describes methodologies for making a highly cost-effective, chemically defined medium for culturing hiPSCs we call B8 medium. The typical cost of B8 medium is US$10 per liter, which with modifications included here is more affordable than standard media. We provide simple protocols for making B8 supplement aliquots, making the basal media DMEM/F12, Matrigel-coated plates, thawing, passaging, culturing, and cryopreserving hiPSCs. We show typical differentiation results and provide a comprehensive troubleshooting guide. For complete details on the use and execution of this protocol, please refer to Kuo et al. (2020).
Topics & Concepts
TroubleshootingMatrigelProtocol (science)Induced pluripotent stem cellComputer scienceHuman Induced Pluripotent Stem CellsChemistryBiologyCell cultureOperating systemBiochemistryMedicineGeneticsEmbryonic stem cellPathologyGeneAlternative medicinePluripotent Stem Cells Research3D Printing in Biomedical ResearchCRISPR and Genetic Engineering