Comprehensive phage display viral antibody profiling using VirScan: potential applications in chronic immune-mediated disease
Jed Valentiner Shrewsbury, Evangelin Shaloom Vitus, Adam Koziol, Anna Nenarokova, Tine Jess, Rahma Elmahdi
Abstract
Phage immunoprecipitation sequencing (PhIP-Seq) is a high-throughput platform that uses programmable phage display for serology. VirScan, a specific PhIP-Seq library encoding viral peptides from all known human viruses, enables comprehensive quantification of past viral exposures. We review its use in immune-mediated diseases (IMDs), highlighting its utility in identifying viral exposures in the context of IMD development. Finally, we evaluate its potential for precision medicine by integrating it with other large-scale omics data sets.
Topics & Concepts
BiologyPhage displayComputational biologyVirologyImmune systemContext (archaeology)AntibodyProfiling (computer programming)ImmunoprecipitationSerologyImmunologyComputer sciencePaleontologyOperating systemMonoclonal and Polyclonal Antibodies ResearchViral Infections and Immunology ResearchCytomegalovirus and herpesvirus research