Vacuole-Specific Lipid Release for Tracking Intracellular Lipid Metabolism and Transport in <i>Saccharomyces cerevisiae</i>
Vladimir Girik, Suihan Feng, Hanaa Hariri, W. Mike Henne, Howard Riezman
Abstract
. Using this approach, we monitored real-time sphingolipid metabolic flux out of the vacuole by mass spectrometry and found that the endoplasmic reticulum-vacuole-tethering protein Mdm1 facilitated the metabolism of sphingoid bases into ceramides. In addition, we showed that cholesterol, once delivered into yeast using our method, could restore cell proliferation induced by ergosterol deprivation, overcoming the previously described sterol-uptake barrier under aerobic conditions. Together, these data define a new way to study intracellular lipid metabolism and transport from the vacuole in yeast.
Topics & Concepts
VacuoleSaccharomyces cerevisiaeSphingolipidLipid metabolismBiochemistryEndoplasmic reticulumLipid dropletErgosterolYeastIntracellularCell biologyBiologyMetabolismCytoplasmEndoplasmic Reticulum Stress and DiseaseCellular transport and secretionLipid Membrane Structure and Behavior