Antimycobacterial and antibacterial activity of green-synthesized silver and zinc oxide nanoparticles using Diospyros montana L. leaf extract
Bheemanagouda N. Patil, T. C. Taranath, Prashanth Konkal
Abstract
This study investigated the use of Diospyros montana L. leaf extract as a green agent for the synthesis of silver and zinc oxide nanoparticles (NPs). The synthesized NPs were characterized using a UV-Vis spectrophotometer, high-resolution transmission electron microscopy (HR-TEM), powder X-ray diffraction (PXRD), and energy-dispersive X-ray (EDX) spectroscopy. The results revealed that the silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs) exhibited spherical and hexagonal shapes, with sizes ranging from 10 to 35 nm for AgNPs and 18–30 nm for ZnONPs. Furthermore, antibacterial activity tests demonstrated that AgNPs exhibited a significantly higher zone of inhibition (15.00 ± 0.00 mm) against Pseudomonas aeruginosa at a concentration of 400 µg/mL, outperforming the antibiotic erythromycin. Additionally, both silver and zinc oxide NPs effectively inhibited the growth of Mycobacterium tuberculosis at concentrations of 25 and 50 µg/mL, respectively. These findings suggest that D. montana leaf extract enables the sustainable production of nanoparticles with remarkable antibacterial and anti-tubercular potential, opening new avenues for innovative biomedical solutions.