Effect of Ascorbic Acid on Differentiation, Secretome and Stemness of Stem Cells from Human Exfoliated Deciduous Tooth (SHEDs)
Shilpa Bhandi, Ahmed Alkahtani, Mohammed Mashyakhy, Abdulaziz S. Abumelha, Nassreen H. Albar, Apathsakayan Renugalakshmi, Mazen F. Alkahtany, Ali Robaian, Asma Almeslet, Vikrant R. Patil, Saranya Varadarajan, Thodur Madapusi Balaji, Rodolfo Reda, Luca Testarelli, Shankargouda Patil
Abstract
Stem cells from human exfoliated deciduous teeth (SHEDs) are considered a type of mesenchymal stem cells (MSCs) because of their unique origin from the neural crest. SHEDs can self-renewal and multi-lineage differentiation with the ability to differentiate into odontoblasts, osteoblast, chondrocytes, neuronal cells, hepatocytes, adipocytes, etc. They are emerging as an ideal source of MSCs because of their easy availability and extraordinary cell number. Ascorbic acid, or vitamin C, has many cell-based applications, such as bone regeneration, osteoblastic differentiation, or extracellular matrix production. It also impacts stem cell plasticity and the ability to sustain pluripotent activity. In this study, we evaluate the effects of ascorbic acid on stemness, paracrine secretion, and differentiation into osteoblast, chondrocytes, and adipocytes. SHEDs displayed enhanced multifaceted activity, which may have applications in regenerative therapy.