Efficient polyethylene terephthalate biodegradation by an engineered <i>Ideonella sakaiensis</i> PETase with a fixed substrate-binding W156 residue
Qingdian Yin, Jiaxing Zhang, Sen Ma, Tao Gu, Mengfan Wang, Shengping You, Sheng Ye, Rongxin Su, Yaxin Wang, Wei Qi
Abstract
The S92P/D157A variant of Ideonella sakaiensis PETase ( Is PETase) showed significantly enhanced thermostability and PET degradation activity. The W156 residue of the variant was fixed in the substrate-binding conformation.
Topics & Concepts
Polyethylene terephthalateResidue (chemistry)ThermostabilityBiodegradationSubstrate (aquarium)ChemistryDegradation (telecommunications)Substrate specificityPolymer chemistryMaterials scienceOrganic chemistryEnzymeComposite materialBiologyTelecommunicationsComputer scienceEcologyMicroplastics and Plastic PollutionGraphene and Nanomaterials Applicationsbiodegradable polymer synthesis and properties