PAXX binding to the NHEJ machinery explains functional redundancy with XLF
Murielle Seif-El-Dahan, Antonia Kefala-Stavridi, Philippe Frit, Steven W. Hardwick, Dimitri Y. Chirgadze, Taiana Maia De Oliviera, Jessica Andréani, Sébastien Britton, Nadia Barboule, Madeleine Bossaert, Arun Prasad Pandurangan, Katheryn Meek, Tom L. Blundell, Virginie Ropars, Patrick Calsou, Jean‐Baptiste Charbonnier, Amanda K. Chaplin
Abstract
Nonhomologous end joining is a critical mechanism that repairs DNA double-strand breaks in human cells. In this work, we address the structural and functional role of the accessory protein PAXX [paralog of x-ray repair cross-complementing protein 4 (XRCC4) and XRCC4-like factor (XLF)] in this mechanism. Here, we report high-resolution cryo-electron microscopy (cryo-EM) and x-ray crystallography structures of the PAXX C-terminal Ku-binding motif bound to Ku70/80 and cryo-EM structures of PAXX bound to two alternate DNA-dependent protein kinase (DNA-PK) end-bridging dimers, mediated by either Ku80 or XLF. We identify residues critical for the Ku70/PAXX interaction in vitro and in cells. We demonstrate that PAXX and XLF can bind simultaneously to the Ku heterodimer and act as structural bridges in alternate forms of DNA-PK dimers. Last, we show that engagement of both proteins provides a complementary advantage for DNA end synapsis and end joining in cells.