Development of a fluorescent probe library enabling efficient screening of tumour-imaging probes based on discovery of biomarker enzymatic activities
Yugo Kuriki, Takafusa Yoshioka, Mako Kamiya, Toru Komatsu, Hiroyuki Takamaru, Kyohhei Fujita, Hirohisa Iwaki, Aika Nanjo, Yuki Akagi, Kohei Takeshita, Haruaki Hino, Rumi Hino, Ryosuke Kojima, Tasuku Ueno, Kenjiro Hanaoka, Seiichiro Abe, Yutaka Saito, Jun Nakajima, Yasuteru Urano
Abstract
screening of freshly resected clinical specimens from lung or gastric cancer patients, and found promising probes for tumour-selective visualization. Further, we identified two target enzymes as novel biomarker enzymes for discriminating between tumour and non-tumour tissues. This library-based approach is expected to be an efficient tool to develop tumour-imaging probes and to discover new biomarker enzyme activities for various tumours and other diseases.
Topics & Concepts
BiomarkerEx vivoMolecular imagingIn vivoBiomarker discoveryFluorescenceEnzymeCancer biomarkersComputational biologyProteasesFluorescence-lifetime imaging microscopyChemistryCancerIn vitroBiochemistryBiologyProteomicsBiotechnologyGeneticsQuantum mechanicsPhysicsGenePeptidase Inhibition and AnalysisClick Chemistry and ApplicationsChemical Synthesis and Analysis