Salmonella enterica Serovar Typhimurium SPI-1 and SPI-2 Shape the Global Transcriptional Landscape in a Human Intestinal Organoid Model System
Anna-Lisa E. Lawrence, Basel H. Abuaita, Ryan P. Berger, David R. Hill, Sha Huang, Veda K. Yadagiri, Brooke Bons, Courtney Fields, Christiane E. Wobus, Jason R. Spence, Vincent B. Young, Mary O’Riordan
Abstract
Typhimurium upregulates proinflammatory pathways independently of either secretion system, while the downregulation of the host cell cycle pathways relies on both SPI-1 and SPI-2. These findings lay the groundwork for future studies investigating how SPI-1- and SPI-2-driven host responses affect infection outcome and show the potential of this model to study host-pathogen interactions with other serovars to understand how initial interactions with the intestinal epithelium may affect pathogenesis.
Topics & Concepts
Salmonella entericaSalmonellaOrganoidSerotypeMicrobiologyMicrobiomeBiologyHuman healthComputational biologyBacteriaVirologyBioinformaticsMedicineGeneticsEnvironmental healthCancer Research and TreatmentsSalmonella and Campylobacter epidemiologyBacterial Genetics and Biotechnology