A systematic study on the discrepancy of fluorescence properties between in solutions and in cells: super-bright, environment-insensitive benzocoumarin dyes
Ye Jin Reo, Yong Woong Jun, Seo Won Cho, Jinseong Jeon, Hajung Roh, Subhankar Singha, Mingchong Dai, Sourav Sarkar, Hye Rim Kim, Sojeong Kim, Yusung Jin, Yun Lim Jung, Yun Jae Yang, Changil Ban, Jinmyoung Joo, Kyo Han Ahn
Abstract
The benzocoumarin dyes fluoresce negligibly in aqueous media but very strongly in cells, whereas representative conventional dyes display contrasting behaviour; the distinct emission behaviour of the fluorophores in organic solutions, in aqueous media, and in cell convinces the uniqueness of the cellular environment. The in cellulo superbright benzocoumarins also reveal an environment-insensitive emission behaviour, which is required for the reliable analysis via ratiometric imaging.
Topics & Concepts
FluorescenceAqueous solutionSpace (punctuation)Aqueous mediumChemical physicsBuffer (optical fiber)Aggregation-induced emissionMaterials scienceNanotechnologyChemistryBiological systemPhysicsOpticsComputer scienceTelecommunicationsPhysical chemistryBiologyOperating systemPhotochromic and Fluorescence ChemistryAdvanced Fluorescence Microscopy TechniquesMolecular Sensors and Ion Detection