Post column infusion of an internal standard into LC-FT-ICR MS enables semi-quantitative comparison of dissolved organic matter in original samples
Rebecca Rodrigues Matos, Elaine K. Jennings, Jan Kaesler, Thorsten Reemtsma, Boris Koch, Oliver J. Lechtenfeld
Abstract
> 0.9 for 98% of detected molecular formulas across retention times). Importantly, PCI-IS could compensate for 80% of matrix effects across an environmental gradient of DOM composition from groundwater to surface water. This enabled studying the ionization efficiency of DOM isomers and linking the observed differences to the biogeochemical sources. With PCI-IS original, non-extracted DOM samples can be analysed by LC-FT-ICR MS without carbon load adjustment, and mass peak intensities can be reliably used to semi-quantitatively compare isomer abundances between compositionally similar DOM samples.
Topics & Concepts
Dissolved organic carbonChromatographyChemistryColumn (typography)Organic matterEnvironmental chemistryMathematicsOrganic chemistryConnection (principal bundle)GeometryAnalytical chemistry methods developmentMass Spectrometry Techniques and ApplicationsAdvanced Chemical Sensor Technologies