The Pel polysaccharide is predominantly composed of a dimeric repeat of α-1,4 linked galactosamine and N-acetylgalactosamine
François Le Mauff, Erum Razvi, Courtney Reichhardt, Piyanka Sivarajah, Matthew R. Parsek, P. Lynne Howell, Donald C. Sheppard
Abstract
Abstract The genetic capacity to synthesize the biofilm matrix exopolysaccharide Pel is widespread among Gram-negative and Gram-positive bacteria. However, its exact chemical structure has been challenging to determine. Using a Pseudomonas aeruginosa strain engineered to overproduce Pel, improvements to the isolation procedure, and selective hydrolysis with the glycoside hydrolase PelA h , we demonstrate that Pel is a partially de- N- acetylated linear polymer of α-1,4- N- acetylgalactosamine comprised predominantly of dimeric repeats of galactosamine and N- acetylgalactosamine.
Topics & Concepts
GalactosaminePolysaccharideHydrolysisChemistryStrain (injury)BiofilmBacteriaGlycoside hydrolaseBiochemistryPseudomonas aeruginosaMicrobiologyAcetylationBiologyGeneGalactoseGeneticsAnatomyBacterial biofilms and quorum sensingBacterial Genetics and BiotechnologyOral microbiology and periodontitis research