Effect of Dry Eye Disease on the Kinetics of Lacrimal Gland Dendritic Cells as Visualized by Intravital Multi-Photon Microscopy
Gustavo Ortiz, Cecilia Chao, Arsia Jamali, Yashar Seyed‐Razavi, Brendan Kenyon, Deshea L Harris, Driss Zoukhri, Pedram Hamrah
Abstract
< 0.001). Our IV-MPM study sheds light into the 3D morphological alterations and cDC kinetics in the LG during DED. While in naïve LGs, cDCs exhibit a more dendritic morphology and are less motile, they became more spherical with enhanced motility during DED. This study shows that IV-MPM represents a robust tool to study immune cell trafficking and kinetics in the LG, which might elucidate cellular alterations in immunological diseases, such as DED.
Topics & Concepts
Intravital microscopyImmune systemPathologyImmunofluorescenceSecretionChemistryConfocal microscopyPeripheral blood mononuclear cellDendritic cellMedicineImmunologyInternal medicineEndocrinologyBiologyAntibodyCell biologyMicrocirculationIn vitroBiochemistryOcular Surface and Contact LensAllergic Rhinitis and SensitizationDermatology and Skin Diseases