Engineered Biosynthesis of Fungal 4-Quinolone Natural Products
Mengting Liu, Masao Ōhashi, Yi Tang
Abstract
Quinolone-containing natural products are widely found in bacteria, fungi, and plants. The fungal quinolactacins, which are N-methyl-4-quinolones, display a wide spectrum of biological activities. Here we uncovered a concise nonribosomal peptide synthetase pathway involved in quinolactacin A biosynthesis from Penicillium by using heterologous reconstitution and in vitro enzymatic synthesis. The N-desmethyl analog of quinolactacin A was accessed through the construction of a hybrid bacterial and fungi pathway in the heterologous host.
Topics & Concepts
Nonribosomal peptideQuinoloneHeterologousBiosynthesisChemistryHeterologous expressionPenicilliumEnzymeBacteriaBiochemistryIn vitroMicrobiologyStereochemistryBiologyAntibioticsRecombinant DNAGeneFood scienceGeneticsMicrobial Natural Products and BiosynthesisPlant-Microbe Interactions and ImmunityBiochemical and Structural Characterization