BLI‐MS: Combining biolayer interferometry and mass spectrometry
Vincent Jung, Kévin Roger, Cérina Chhuon, Louise Pannetier, Joanna Lipecka, Josué Sulub Gomez, Pascal Chappert, Alain Charbit, Ida Chiara Guerrera
Abstract
Biolayer interferometry (BLI) is a technology which allows to study the affinity between two interacting macro-molecules and to visualize their kinetic of interaction in real time. In this work, we combine BLI interaction measurement with mass spectrometry in order to identify the proteins interacting with the bait. We provide for the first time the proof of concept of the feasibility of BLI-MS in complex biological mixtures.
Topics & Concepts
Mass spectrometryChemistryInterferometryComputational biologyChromatographyPhysicsBiologyOpticsAdvanced Biosensing Techniques and ApplicationsViral Infectious Diseases and Gene Expression in InsectsAdvanced Proteomics Techniques and Applications