Characterization of <i>Sphingobacterium</i> sp. Ab3 Lipase and Its Coexpression with LEA Peptides
You Kiat Ng, Shinya Ikeno, Ammar Khazaal Kadhim Almansoori, Ibrahim Muhammad, Rashidah Abdul Rahim
Abstract
The mechanism of action of LEA proteins has remained unknown to many; in this study we reveal their presence and improved protein expression due to the molecular shielding effect reported by others. This paper should be regarded as a useful example of using such proteins to influence an existing expression system to produce difficult-to-express proteins.
Topics & Concepts
LipaseEnzymeCofactorBiochemistryBacteriaChemistryChromatographyBiologyGeneticsEnzyme Catalysis and ImmobilizationMicrobial Metabolic Engineering and BioproductionPancreatic function and diabetes