Detection of CWD prions in naturally infected white-tailed deer fetuses and gestational tissues by PMCA
Francisca Bravo‐Risi, Paulina Soto, Thomas Eckland, Robert O. Dittmar, Santiago Ramírez, Celso S. G. Catumbela, Claudio Soto, Mitch Lockwood, Tracy A. Nichols, Rodrigo Morales
Abstract
Chronic wasting disease (CWD) is a prevalent prion disease affecting cervids. CWD is thought to be transmitted through direct animal contact or by indirect exposure to contaminated environmental fomites. Other mechanisms of propagation such as vertical and maternal transmissions have also been suggested using naturally and experimentally infected animals. Here, we describe the detection of CWD prions in naturally-infected, farmed white-tailed deer (WTD) fetal tissues using the Protein Misfolding Cyclic Amplification (PMCA) technique. Prion seeding activity was identified in a variety of gestational and fetal tissues. Future studies should demonstrate if prions present in fetuses are at sufficient quantities to cause CWD after birth. This data confirms previous findings in other animal species and furthers vertical transmission as a relevant mechanism of CWD dissemination.