FRET-Based Assay for the Quantification of Extracellular Vesicles and Other Vesicles of Complex Composition
Konrad Thorsteinsson, Erik Olsén, Eneas Schmidt, Hudson Pace, Marta Bally
Abstract
vesicles/mL, using as little as 60 μL of the sample. The assay precision was comparable to that of a commercial nanoparticle tracking analysis system. While its limit of detection was slightly higher, the FRET assay is superior for the detection of small vesicles, as its performance is vesicle-size-independent. Taken together, the FRET assay is a simple, robust, and versatile method for the quantification of a variety of purified vesicle samples.
Topics & Concepts
VesicleFörster resonance energy transferChemistryLiposomeMembraneBiophysicsVesicle fusionExtracellular vesiclesSonicationExtracellular vesicleLipid bilayerNanoparticle tracking analysisChromatographyBiochemistryFluorescenceMicrovesiclesSynaptic vesicleCell biologyBiologyQuantum mechanicsPhysicsmicroRNAGeneExtracellular vesicles in diseaseBacterial Infections and VaccinesLipid Membrane Structure and Behavior