Bioefficacy of Zinc oxide nanoparticle synthesis and their Biological, Environmental applications from Eranthemum roseum
Ramachandran Adhavan, Kuppusamy Selvam, Palanisamy Prakash, Peraman Manimegalai, Dharmalingam Kirubakaran, Muthugounder Subramanian Shivakumar
Abstract
Synthesis of metal oxide nanoparticles using medicinal plant increasing rapidly due to its eco-friendly to environment. In this study Zinc oxide nanoparticle is synthesized using the leaf extract of plant E. roseum. Synthesized NPs was characterized using UV- Vis Spectroscopy analysis where the peak observed at 374 nm with band gap of 2.5 eV, FTIR and XRD analysis validate pure hexagonal structure, Spherical shape of NPs was confirmed by SEM with EDX analysis and main compounds are zinc 75% and oxygen are 22%. Transmission Electron Microscopy Analysis confirms the oval shaped ZnO NPs Biological activity of E. roseum ZnO NPs such as antioxidant assay DPPH, ABTS, hydroxyl radical and reducing power activity shows good inhibition against free radicals. The In-vitro Hypoglycemic effects has maximum inhibition of 96% on α- amylase activity and 87% on α- Glycosidase activity. Anti-inflammatory activity recorded 93% maximum inhibition at Albumin denaturation assay and 75% at Membrane stabilization assay. E. roseum ZnO NPs shows 67.79% on HeGp2 Anti-proliferative cells line. AO/EtBr staining assays confirms the apoptosis effect. Larvicidal activity shows highest mortality of 98.44% on species C. quinquefasciatus. Photocatalytic dyedegradation of Methylene blue dye shows 65% of dye degradation. • This study confirmed the green synthesis of ZnONPs derived from Eranthemum roseum. • The synthesized ZnONPs were characterized through various techniques, including UV, FTIR, XRD, SEM with EDAX and TEM analysis. • The green-synthesized ZnONPs exhibited remarkable antibacterial, antioxidant, anti-inflammatory and antidiabetic properties. • The anticancer potential of the synthesized ZnONPs was assessed using HeGp2 and AO/EtBr staining assays.