Optimisation of methodology for whole genome sequencing of Measles Virus directly from patient specimens
Helene Schulz, Joanne Hiebert, Jasmine Rae Frost, Elizabeth McLachlan, Alberto Severini
Abstract
In an era of decreasing genetic diversity of Measles Virus (MeV), effective surveillance requires a higher-resolution genotyping method or whole genome sequencing (WGS) to document elimination. Through optimization of MeV WGS protocol, we developed a MeV-specific probe enrichment method that allows next generation sequencing from clinical specimens. With the probe enrichment method, 70% of specimens can be sequenced at a read depth of greater than 10 reads with minimal off-target sequences.
Topics & Concepts
BiologyGenotypingMeasles virusGenomeWhole genome sequencingDNA sequencingComputational biologyVirusGeneticsVirologyMeaslesGenotypeGeneVaccinationVirology and Viral Diseasesvaccines and immunoinformatics approachesAutism Spectrum Disorder Research