Multi-Dimensional High-Performance Liquid Chromatographic Determination of Chiral Amino Acids and Related Compounds in Real World Samples
Chiharu Ishii, Aogu Furusho, Chin‐Ling Hsieh, Kenji Hamase
Abstract
Chiral amino acid analysis, especially the determination of trace levels of D-enantiomers, is currently gathering attention in a variety of research areas including the food/clinical sciences. These D-amino acids had long been believed to be absent in the higher animals. However, by the advances of analytical technologies, some of the D-enantiomers are found in mammals including humans and increasingly recognized as novel physiologically-active substances and/or biomarkers. For the determination of these D-amino acids and related compounds in real world samples, utilization of sensitive and selective methods is essential and multi-dimensional HPLC is one of the straightforward approaches. In the present review, two/three-dimensional HPLC methods and biological/medical applications focusing on our current studies are summarized.