Development of a Parallel Reaction Monitoring Mass Spectrometry Assay for the Detection of SARS-CoV-2 Spike Glycoprotein and Nucleoprotein
Lisa H. Cazares, Raghothama Chaerkady, Shao Huan Samuel Weng, Chelsea C. Boo, Raffaello Cimbro, Hsiang-En Hsu, Sarav Rajan, William F. Dall’Acqua, Lori A. Clarke, Kuishu Ren, Patrick M. McTamney, Nicole Kallewaard-LeLay, Mahboobe Ghaedi, Yasuhiro Ikeda, Sonja Hess
Abstract
viral particles/mL, making it an attractive alternative to RT-PCR assays. Potentially, mass spectrometry-based methods for viral antigen detection may deliver higher throughput and could serve as a complementary diagnostic tool to RT-PCR. Furthermore, this assay could be used to evaluate the presence of SARS-CoV-2 in archived or recently collected biological fluids, in vitro-derived research materials, and wastewater samples.
Topics & Concepts
NucleoproteinChemistryMass spectrometryNucleic acidDetection limitVirologyChromatographyMolecular biologyDNABiochemistryBiologySARS-CoV-2 detection and testingSARS-CoV-2 and COVID-19 ResearchBiosensors and Analytical Detection