Influenza A(H5N1) shedding in air corresponds to transmissibility in mammals
Ilona I. Tosheva, Fabien Filaire, Willemijn F. Rijnink, Dennis de Meulder, Bianca van Kekem, Theo M. Bestebroer, Mathis Funk, Monique I. Spronken, C. Joaquín Cáceres, Daniel R. Pérez, Mathilde Richard, Marion Koopmans, Pieter L. A. Fraaij, Ron A. M. Fouchier, Sander Herfst
Abstract
Abstract An increase in spillover events of highly pathogenic avian influenza A(H5N1) viruses to mammals suggests selection of viruses that transmit well in mammals. Here we use air-sampling devices to continuously sample infectious influenza viruses expelled by experimentally infected ferrets. The resulting quantitative virus shedding kinetics data resembled ferret-to-ferret transmission studies and indicated that the absence of transmission observed for earlier A(H5N1) viruses was due to a lack of infectious virus shedding in the air, rather than the absence of necessary mammalian adaptation mutations. Whereas infectious human A(H1N1 pdm ) virus was efficiently shed in the air, infectious 2005 zoonotic and 2024 bovine A(H5N1) viruses were not detected in the air. By contrast, shedding of infectious virus was observed for 1 out of 4 ferrets infected with a 2022 European polecat A(H5N1) virus and a 2024 A(H5N1) virus isolated from a dairy farm worker.