Antagonistic Mechanism Analysis of <i>Bacillus velezensis</i> JLU-1, a Biocontrol Agent of Rice Pathogen <i>Magnaporthe oryzae</i>
Longhao Su, Jiyue Zhang, Jinyu Fan, Dan Li, Meixi Zhao, Yi‐Chi Wang, Hongyu Pan, Леи Жао, Xianghui Zhang
Abstract
Magnaporthe oryzae, the causal agent of rice blast, is a fungal disease pathogen. Bacillus spp. have emerged as the most promising biological control agent alternative to chemical fungicides. In this study, the bacterial strain JLU-1 with significant antagonistic activity isolated from the rhizosphere soil of rice was identified as Bacillus velezensis through whole-genome sequencing, average nucleotide identity analysis, and 16S rRNA gene sequencing. Twelve gene clusters for secondary metabolite synthesis were identified in JLU-1. Furthermore, 3 secondary metabolites were identified in JLU-1, and the antagonistic effect of secondary metabolites against fungal pathogens was confirmed. Exposure to JLU-1 reduced the virulence of M. oryzae, and JLU-1 has the ability to induce the reactive oxygen species production of rice and improve the salt tolerance of rice. All of these results indicated that JLU-1 and its secondary metabolites have the promising potential to be developed into a biocontrol agent to control fungal diseases.